Detection and identification of the two Candidatus Liberobacter species associated with citrus huanglongbing by PCR amplification of ribosomal protein genes of theβ operon
Identifieur interne : 000209 ( France/Analysis ); précédent : 000208; suivant : 000210Detection and identification of the two Candidatus Liberobacter species associated with citrus huanglongbing by PCR amplification of ribosomal protein genes of theβ operon
Auteurs : A. Hocquellet [France] ; P. Toorawa [France] ; J.-M Bové [France] ; M. Garnier [France]Source :
- Molecular and Cellular Probes [ 0890-8508 ] ; 1999.
English descriptors
Abstract
Huanglongbing, previously called greening, is a destructive disease of citrus in Asia and Africa. It is caused by an uncultured, phloem-restricted bacterium for which two species Candidatus Liberobacter asiaticum andCandidatus Liberobacter africanum have been characterized. In 1996, a polymerase chain reaction (PCR) detection method based on the amplification of the 16S ribosomal operon was developed and proved to be efficient for the detection of both liberobacter species. However, in order to distinguish between Candidatus Liberobacter asiaticum and Candidatus Liberobacter africanum, digestion of the amplified DNA with Xba I was required. We have now developed a new PCR detection method based on the amplification of ribosomal protein genes which allows direct identification of the liberobacter species by the size of the amplified DNA. This PCR method is as specific, and at least as sensitive as the previously described one for detection of the two liberobacter species
Url:
DOI: 10.1006/mcpr.1999.0263
Affiliations:
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ISTEX:93D5329C5371F013D59471A930A5C29DC183F6E1Le document en format XML
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It is caused by an uncultured, phloem-restricted bacterium for which two species Candidatus Liberobacter asiaticum andCandidatus Liberobacter africanum have been characterized. In 1996, a polymerase chain reaction (PCR) detection method based on the amplification of the 16S ribosomal operon was developed and proved to be efficient for the detection of both liberobacter species. However, in order to distinguish between Candidatus Liberobacter asiaticum and Candidatus Liberobacter africanum, digestion of the amplified DNA with Xba I was required. We have now developed a new PCR detection method based on the amplification of ribosomal protein genes which allows direct identification of the liberobacter species by the size of the amplified DNA. 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